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Xenopus oocytes에서의 electro-potential 측정장치
The Roboocyte는 새로운 개념의 HTS(high-throughput screenings)로서 Xenopus oocytes에서 자종적으로
TEVC를 측정하는 장치이며, 현재 8 channels과 16 channels를 이용할수 있다
Technical Specifications:
Main Device (without Perfusion System):
- Dimensions: 390 mm x 360 mm x 310 mm (WxDxH)
- Weight: 34.5 kg
- Line voltage: 100-240 V
ClampAmp:
General
- Newly designed integrated digital TEVC amplifier
- Headstages included
- Operates fully automatically and computer-controlled
- Active bath clamp with two independent reference electrodes
- Acquisition rate: 2 kHz, 1 kHz, 500 Hz, ..., 1 Hz
- Data resolution: 16 bits
- Recommended electrode resistance range: 100 kOhm
to 1 MOhm
Current elctrode output:
- Output range: -32000 nA to + 32000 nA
- Current resolution: 1 nA
- Voltage range: -32 V to + 32 V
- Voltage resolution: 1 mV
Voltage electrode inout:
- Input range: -500 mV to + 500 mV
- Voltage resolution: 0.125 mV
- Clamp voltage setpoint range: -500 mV to + 500 mV
- Clamp voltage setpoint resolution: 1 mV
- Minimum rise time: 1 ms
Performance and Throughput:
- Operates with disposable standard 96 well plates
- Movement time, from well to well: 2 s
- Average time needed to inject 96 oocytes (assumed injection time = 1 s): 5 min
- Positioning accuracy: 20 탆 in X/Y dimensions and 33 탆 in Z dimension
- Injection pressure: 0-3 bar
Perfusion System:
- 8-channel gravity-based pinch valve system with integrated diaphragm pump, flow rate approx. 4 ml/min
- Full integration of an external liquid handler (for example GILSON
222 XL Liquid Handler) via RS32 serial port
- Either system is fully controlled and automated by the Roboocyte software.
- Number of compounds is limited only by oocyte performance.
Software:
- Full automatization and control of all devices and features including perfusion via scripting
- Connection to the Roboocyte via Ethernet
- Operating system: Windows 2000, Windows XP
- Data export in ASCII file format
- Linkage to Microsoft Access database
Accessories:
- Fully installed Dell computer with Ethernet card and 17" flat screen monitor
- Stereo microscope with lights
- Pre-pulled injection micropipettes
- Ready to Use TEVC probes
Manual과 이 장치를 시용한 경우 비교표
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Manual Operation |
The Roboocyte |
| Injections/day |
150 |
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1.000-2.000 |
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| Compounds/year |
500 |
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10.000 |
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| Data points/year |
12.500 |
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500.000 |
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(based on the expertise of Bayer AG laboratories) |
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