SERCA
  *   SERCA resides in the sarcoplasmic reticulum (SR) within muscle cells. It is a Ca2+ ATPase that transfers Ca2+ from the cytosol of the cell to the lumen of the SR at the expense of ATP hydrolysis during muscle relaxation.
    There are 3 major domains on the cytoplasmic face of SERCA: the phosphorylation and nucleotide-binding domains, which form the catalytic site, and the actuator domain, which is involved in the transmission of major conformational changes.

  *   SERCA is normally inhibited by the defective phosphorylation of protein phospholamban, with which it is closely associated. Another protein, calsequestrin, binds calcium within the SR and helps to reduce the concentration of free calcium within the SR, which assists SERCA so that it does not have to pump against such a high concentration gradient. The SR has a much higher concentration of Ca2+ (10,000x) inside when compared to the intracellular Ca2+ concentration.
    The rate at which SERCA moves Ca2+ across the SR membrane can be controlled by phospholamban (PLB/PLN) under β-adrenergic stimulation. Increased β-adrenergic stimulation reduces association between SERCA and PLB by phosphorylation of PLB. When PLB is associated with SERCA, the rate of Ca2+ movement is reduced, upon dissociation of PLB Ca2+ movement increases.

  *   There are three genes for the SERCA protein, which display different patterns of cell/tissue expression. SERCA1 (ATP2A1) is expressed in fast-twitch skeletal muscle, SERCA2 (ATP2A2) is expressed in cardiac, smooth and slow-twitch skeletal muscle, and SERCA3 (ATP2A3) which is expressed more ubiquitously. Each gene encodes a protein of approximately 110kDa.

 

anti-SERCA B/4H3 Antibody
Cat. No.: A010-22AP
Description: Affinity purified Mouse monoclonal antibody to skeletal muscle SERCA1
Unit Volume: 50 ul
Tested Applications: Western Blot (1:5000 dilution), IHC Microscopy (1:200 dilution). Not yet tested in other applications, therefore, optimal dilutions/ concentrations should be determined by the user.
Antibody form: Lyophilised Protein A Affinity Purified Mouse monoclonal IgG
Species recognition: Antibody tested on Rat and rabbit tissue, but immunogenic peptide aligns with Mammalian species, Avian, Amphibian and Fish
anti-SERCA Y/IF4
Cat. No.: A010-21AP
Description: Affinity purified Mouse monoclonal antibody to SERCA isoforms 1 / 2
Unit Volume: 50 ul
Tested Applications: Western Blot (1:2000 dilution), IHC Microscopy (1:50 dilution).
Antibody form: Lyophilised Protein A Affinity Purified Mouse monoclonal IgG
Species recognition: Tested on Rat tissue, but immunogenic peptide aligns with Mammalian, Avian, Amphibian and Fish SERCA
anti-SERCA2 Phospho Serine-38 Ab
Cat. No.: A010-25AP
Description: Affinity purified polyclonal antibody specific to Ser-38 phosphorylated forms of SERCA2.
Unit Volume: 50 ul
Tested Applications: Western Blot (1:1000 dilution)
Antibody form: Lyophilised Affinity Purified IgG.
Species recognition: Epitope sequence exists in SERCA2 from all mammalian species
anti-SERCA2 Phospho Serine-38 anti-serum
Cat. No.: A010-25
Description: Rabbit polyclonal anti-serum to SERCA2 phospho Serine-38
Unit Volume: 20 ul
Tested Applications: Western Blot (1:5000 dilution), ELISA. Not yet tested in other applications, therefore, optimal dilutions/concentrations should be determined by the user.
Antibody form: Lyophilised Rabbit Anti-Serum
Species recognition: Epitope sequence exists in SERCA2 from all mammalian species
anti-SERCA2a anti-serum
Cat. No.: A010-20
Description: Rabbit polyclonal antibody to SERCA2a
Unit volume: 20 ul
Tested Applications: Western blot (1:5000 dilution)
Antibody Form: IgG
Antibody Purity: Raw anti-serum
Species Reactivity: Reacts with SERCA2a from human, rat, mouse, rabbit, canine, and bovine species.
 
      Antibody for Cardiovascular Targets

Phospholamban
Erk
Ryanodine Receptor
CaMK II
Cav1.2
β-dystroglycan
Calcineurin