| HOPE¢ç is the formalin-free fixation technique for molecular pathology and research.
Tissue conservation using the new HOPE¢ç-technique results in paraffin embedded tissue blocks with native structure of the macro-molecules (protein, DNA, RNA) in the tissue conserved.
During HOPE¢ç fixation, tissue samples are incubated in HOPE¢ç I solution, then in HOPE¢ç II solution, followed by a dehydration with acetone and subsequent embedding in paraffin.
For this tissue processing no alcohols or aldehydes (formalin, glutaraldehyde) are used. As a consequence, there is no cross-linking of macro-molecular structures during the process. This opens up new opportunities to characterize tissues out of paraffin blocks.
Many "cryo-type" antibodies are working on HOPE¢ç fixed tissue sections and many antibodies are working without any heat pretreatment or proteolytic digestion steps often necessary for formalin fixed tissue sections.
There is no "over-fixation" with HOPE¢ç, as is known with formalin, when keeping the tissue specimen in the fixation solution. This results in a more standardized fixation process.
RNA, DNA and proteins are conserved in high quality, thus making subsequent applications possible like e.g. PCR, RT-PCR, Northern-Blot, Immunohistochemistry (IHC), in situ hybridization (ISH), Western-Blot (WB) and many others, out of the same tissue blocks.