HOPE ( formalin-free fixation )

    HOPE¢ç is the formalin-free fixation technique for molecular pathology and research.

    Tissue conservation using the new HOPE¢ç-technique results in paraffin embedded tissue blocks with native structure of the macro-molecules (protein, DNA, RNA) in the tissue conserved.

    During HOPE¢ç fixation, tissue samples are incubated in HOPE¢ç I solution, then in HOPE¢ç II solution, followed by a dehydration with acetone and subsequent embedding in paraffin.

    For this tissue processing no alcohols or aldehydes (formalin, glutaraldehyde) are used. As a consequence, there is no cross-linking of macro-molecular structures during the process. This opens up new opportunities to characterize tissues out of paraffin blocks.

    Many "cryo-type" antibodies are working on HOPE¢ç fixed tissue sections and many antibodies are working without any heat pretreatment or proteolytic digestion steps often necessary for formalin fixed tissue sections.

    There is no "over-fixation" with HOPE¢ç, as is known with formalin, when keeping the tissue specimen in the fixation solution. This results in a more standardized fixation process.

    RNA, DNA and proteins are conserved in high quality, thus making subsequent applications possible like e.g. PCR, RT-PCR, Northern-Blot, Immunohistochemistry (IHC), in situ hybridization (ISH), Western-Blot (WB) and many others, out of the same tissue blocks.

Items Package Catalog Remark
  HOPE¢ç I Solution (100 fixations) 2 kg BM-PL003S2K Tissue embedding
Part of the HOPE¢ç tissue fixation system. 1 x 2 kg bag for about 100 paraffin blocks.
  HOPE¢ç Trial-Kit 1 kit HL007R010SG Tissue fixation, IHC, ISH
For easy starting we offer a HOPE¢ç Trail Kit with 100 ml HOPE¢ç I, 1ml HOPE¢ç II and 500 g Low Melting Paraffin sufficient for fixation of 20 tissue samples.
  HOPE¢ç II Solution 1ml BM-HL002C001 Tissue fixation, IHC, ISH
For fixing tissue according to the HOPE¢ç standard fixation protocol, HOPE¢ç I and HOPE¢ç II solution (catalog nr. BM-HL002C001), acetone and Low Melting Point Paraffin (52-54¡ÆC, catalog nr. BM-PL0003S2K ) are required.
  HOPE¢ç I Solution (100 fixations) 500 ml BM-HL001R500 Tissue fixation, IHC, ISH
HOPE¢ç = Hepes-glutamic acid buffer mediated Organic solvent Protection Effect - is a new formalin-free tissue conservation technique for tissue fixation
  HOPE¢ç I Solution (500 fixations) 2.5 L BM-HL001R2500 Tissue fixation, IHC, ISH
HOPE¢ç = Hepes-glutamic acid buffer mediated Organic solvent Protection Effect - is a new formalin-free tissue conservation technique for tissue fixation
  HOPE¢ç I Solution (2000 fixations) 10L BM-HL001B10 Tissue fixation, IHC, ISH
HOPE¢ç = Hepes-glutamic acid buffer mediated Organic solvent Protection Effect - is a new formalin-free tissue conservation technique for tissue fixation