Affinity Chromatography Media

  • N6- (2- Aminoethyl)adenosine- 3', 5'- cyclic monophosphate, immobilized on agarose gel ( 6-AE-cAMP-Agarose )
  • N6- (2- Aminoethyl)adenosine- 3', 5'- cyclic monophosphorothioate, Sp- isomer, immobilized on agarose gel ( Sp-6-AE-cAMPS-Agarose )
  • 8- (2- Aminoethylamino)adenosine- 3', 5'- cyclic monophosphate, immobilized on agarose ( 8-AEA-cAMP-Agarose )
  • 2- (2- Aminoethylamino)adenosine- 3', 5'- cyclic monophosphate, immobilized on agarose gel ( 2-AEA-cAMP-Agarose )
  • 8- (2- Aminoethylamino)adenosine- 3', 5'- cyclic monophosphorothioate, Rp- isomer, immobilized on agarose ( Rp-8-AEA-cAMPS-Agarose )
  • 8- (2- Aminoethylamino)adenosine- 3', 5'- cyclic monophosphorothioate, Sp- isomer, immobilized on agarose ( Sp-8-AEA-cAMPS-Agarose )
  • 2- (2- Aminoethylamino)adenosine- 3', 5'- cyclic monophosphorothioate, Sp- isomer, immobilized on agarose gel ( Sp-2-AEA-cAMPS-Agarose )
  • 2'- O- (6- Aminoethylcarbamoyl)adenosine- 3', 5'- cyclic monophosphate, immobilized on agarose gel ( 2'-AEC-cAMP-Agarose )
  • 2'- O- (6- Aminoethylcarbamoyl)adenosine- 3', 5'- cyclic monophosphorothioate, Sp- isomer, immobilized on agarose gel ( Sp-2'-AHC-cAMPS-Agarose )
  • 8- (2- Aminoethylthio)guanosine- 3', 5'- cyclic monophosphate, immobilized on agarose ( 8-AET-cGMP-Agarose )
  • 8- (2- Aminoethylthio)guanosine- 3', 5'- cyclic monophosphorothioate, Rp- isomer, immobilized on agarose ( Rp-8-AET-cGMPS-Agarose )
  • 8- (2- Aminoethylthio)guanosine- 3', 5'- cyclic monophosphorothioate, Sp- isomer, immobilized on agarose ( Sp-8-AET-cGMPS-Agarose )
  • N6- (6- Aminohexyl)adenosine- 3', 5'- cyclic monophosphate, immobilized on agarose gel ( 6-AH-cAMP Agarose )
  • N6- (6- Aminohexyl)adenosine- 3', 5'- cyclic monophosphorothioate, Sp- isomer, immobilized on agarose gel ( Sp-6-AH-cAMPS-Agarose )
  • N2- (6- Aminohexyl)guanosine- 3', 5'- cyclic monophosphate, immobilized on agarose ( 2-AH-cGMP Agarose )
  • 8- (6- Aminohexylamino)- 2'- O- methyladenosine- 3', 5'- cylic monophosphate, immobilized on agarose ( 8-AHA-2'-O-Me-cAMP-Agarose )
  • 2- (6- Aminohexylamino)adenosine- 3', 5'- cyclic monophosphate, immobilized on agarose ( 2-AHA-cAMP-Agarose )
  • 8- (6- Aminohexylamino)adenosine- 3', 5'- cyclic monophosphate, immobilized on agarose gel ( 8-AHA-cAMP Agarose )
  • 8- (6- Aminohexylamino)adenosine- 3', 5'- cyclic monophosphorothioate, Rp- isomer, immobilized on agarose ( Rp-8-AHA-cAMPS-Agarose )
  • 8- (6- Aminohexylamino)adenosine- 3', 5'- cyclic monophosphorothioate, Sp- isomer, immobilized on agarose gel ( Sp-8-AHA-cAMPS-Agarose )
  • 2- (6- Aminohexylamino)adenosine- 3', 5'- cyclic monophosphorothioate, Sp- isomer, immobilized to agarose gel ( Sp-2-AHA-cAMPS-Agarose )
  • 2'- (6- Aminohexylcarbamoyl)adenosine- 3', 5'- cyclic monophosphate, immobilized on agarose ( 2'-AHC-cAMP-Agarose )
  • 2'- (6- Aminohexylcarbamoyl)adenosine- 3', 5'- cyclic monophosphorothioate, Sp- isomer, immobilized on agarose gel ( Sp-2'-AHC-cAMPS-Agarose )
  • 2'- (6- Aminohexylcarbamoyl)guanosine- 3', 5'- cyclic monophosphate, immobilized on agarose ( 2'-AHC-cGMP-Agarose )
  • 2'- (6- Aminohexylcarbamoyl)guanosine- 3', 5'- cyclic monophosphorothioate, Rp- isomer, immobilized on agarose gel ( Rp-2'-AHC-cGMPS-Agarose )
  • 2'- (6- Aminohexylcarbamoyl)guanosine- 3', 5'- cyclic monophosphorothioate, Sp- isomer, immobilized on agarose gel ( Sp-2'-AHC-cGMPS-Agarose )
  • Ethanolamine immobilized on agarose ( EtOH-NH-Agarose )
     

  • N6- (2- Aminoethyl)adenosine- 3', 5'- cyclic monophosphate, immobilized on agarose gel ( 6-AE-cAMP-Agarose )
       Cyclic AMP immobilized on agarose, used for affinity chromatography of various cyclic nucleotide-responsive proteinssuch as protein
       kinases, phosphodiesterases and others.
       Murashima et al., Biochemistry, 29, 5285 - 5292 (1990).
    A 111 0.6 ml, 2.5 ml, 6.0 ml  

    N6- (2- Aminoethyl)adenosine- 3', 5'- cyclic monophosphorothioate, Sp- isomer, immobilized on agarose gel
       ( Sp-6-AE-cAMPS-Agarose )
       PDE-resistant Sp-cAMPS immobilized on agarose, used for affinity chromatography of various cyclic nucleotide-responsive proteins
       such as protein kinases, phosphodiesterases and others
       Normally all affinity gels are supplied in pre-packed columns. The free beads without a column are available upon request.
    A 109 0.6 ml, 2.5 ml, 6.0 ml  

    8- (2- Aminoethylamino)adenosine- 3', 5'- cyclic monophosphate, immobilized on agarose ( 8-AEA-cAMP-Agarose )
       cyclic AMP immobilized on agarose by an aminoethylamino spacer attached to position 8 of the ligand, used for affinity chromatography
       of various cyclic nucleotide-responsive proteins such as proteinkinases, phosphodiesterases and others.
       Dills et al., Biochemistry, 15, 3724 - 3730 (1976); Scholten et al., J. Proteome Res., 5, 1435 - 1447 (2006).
    A 020 0.6 ml, 2.5 ml, 6.0 ml  

    2- (2- Aminoethylamino)adenosine- 3', 5'- cyclic monophosphate, immobilized on agarose gel
       ( 2-AEA-cAMP-Agarose )
       cyclic AMP immobilized on agarose by an aminoethylamino spacer attached to position 2 of the ligand, used for affinity chromatography
       of various cyclic nucleotide-responsive proteins such as protein kinases, phosphodiesterases and others.
       Scholten et al., J. Proteome Res., 5, 1435 - 1447 (2006).
    A 110 0.6 ml, 2.5 ml, 6.0 ml  

    8- (2- Aminoethylamino)adenosine- 3', 5'- cyclic monophosphorothioate, Rp- isomer, immobilized on agarose
       ( Rp-8-AEA-cAMPS-Agarose )
       Immobilized cAMP Antagonist, used for affinity chromatography
       of various cyclic nucleotide-responsive proteins such as protein kinase A (presumably as its holoenzyme), phosphodiesterases and others.
       Normally all affinity gels are supplied in pre-packed columns. The free beads without a column are available upon request.
    A 007 0.6 ml, 2.5 ml, 6.0 ml  

    8- (2- Aminoethylamino)adenosine- 3', 5'- cyclic monophosphorothioate, Sp- isomer, immobilized on agarose
       ( Sp-8-AEA-cAMPS-Agarose )
       Hydrolysis-resistant Sp-cAMPS immobilized on agarose , used for affinity chromatographyof various cyclic nucleotide-responsive
       proteins such as protein kinase A, phosphodiesterases and others.
       Dills, W.L.et al. Biochem. Biophys. Res. Commun., 62, 70 - 77 (1975)
    A 008 0.6 ml, 2.5 ml, 6.0 ml  

    2- (2- Aminoethylamino)adenosine- 3', 5'- cyclic monophosphorothioate, Sp- isomer, immobilized on agarose gel
       ( Sp-2-AEA-cAMPS-Agarose )
       PDE-resistant Sp-cAMPS immobilized to agarose, used for affinity chromatographyof various cyclic nucleotide-responsive proteins such
       as protein kinase A, phosphodiesterases and others.
       Normally all affinity gels are supplied in pre-packed columns. The free beads without a column are available upon request.
    A 087 0.6 ml, 2.5 ml, 6.0 ml  

    2'- O- (6- Aminoethylcarbamoyl)adenosine- 3', 5'- cyclic monophosphate, immobilized on agarose gel
       ( 2'-AEC-cAMP-Agarose )
       cyclic AMP immobilized on agarose, used for affinity chromatography of various cyclic nucleotide-responsive proteins, especially those
       which tolerate modification of the 2'- ribose hydroxyl group such as some phosphodiesterases.
       Normally all affinity gels are supplied in pre-packed columns. The free beads without a column are available upon request.
    A 112 0.6 ml, 2.5 ml, 6.0 ml  

    8- (2- Aminoethylthio)guanosine- 3', 5'- cyclic monophosphate, immobilized on agarose ( 8-AET-cGMP-Agarose )
       cyclic GMP immobilized on agarose, used for affinity chromatography of various cyclic nucleotide-responsive proteins such as protein
       kinases, phosphodiesterases and others. This type of gel is offered as PDE-resistant version.
       Schlossmann et al., Nature, 404, 197 - 201 (2000); Kim & Park, J. Biochem. Mol. Biol., 36, 299 - 304 (2003); Scholten et al., J. Proteome Res., 5, 1435 - 1447 (2006).
    A 019 0.6 ml, 2.5 ml, 6.0 ml  

    8- (2- Aminoethylthio)guanosine- 3', 5'- cyclic monophosphorothioate, Rp- isomer, immobilized on agarose
       ( Rp-8-AET-cGMPS-Agarose )
       Immobilized cGMP antagonist, used for affinity chromatography of various cyclic nucleotide-responsive proteins such as protein
       kinases, phosphodiesterases and others.
      
    A 009 0.6 ml, 2.5 ml, 6.0 ml  

    8- (2- Aminoethylthio)guanosine- 3', 5'- cyclic monophosphorothioate, Sp- isomer, immobilized on agarose
       ( Sp-8-AET-cGMPS-Agarose )
       Immobilized PDE-resistant PKG agonist, used for affinity chromatography of various cyclic nucleotide-responsive proteins such
       as protein kinases, phosphodiesterases and others.
      
    A 010 0.6 ml, 2.5 ml, 6.0 ml  

    N6- (6- Aminohexyl)adenosine- 3', 5'- cyclic monophosphate, immobilized on agarose gel ( 6-AH-cAMP Agarose )
       cyclic AMP immobilized to agarose, used for affinity chromatography of various cyclic nucleotide-responsive proteins such as protein
       kinases, phosphodiesterases and others.
    Poteet-Smith et al., J. Biol. Chem., 272, 379 - 388 (1997).
    A 036 0.6 ml, 2.5 ml, 6.0 ml  

    N6- (6- Aminohexyl)adenosine- 3', 5'- cyclic monophosphorothioate, Sp- isomer, immobilized on agarose gel
       ( Sp-6-AH-cAMPS-Agarose )
       PDE-resistant Sp-cAMPS immobilized on Agarose, used for affinity chromatography of various cyclic nucleotide-responsive proteins such as protein
       kinases, phosphodiesterases and others.
       Normally all affinity gels are supplied in pre-packed columns. The free beads without a column are available upon request.
    A 108 0.6 ml, 2.5 ml, 6.0 ml  

    N2- (6- Aminohexyl)guanosine- 3', 5'- cyclic monophosphate, immobilized on agarose ( 2-AH-cGMP Agarose )
       Cyclic GMP immobilized to agarose, used for affinity chromatography of various cyclic nucleotide-responsive proteins such as protein
       kinases, phosphodiesterases and others.
       Kim & Park, J. Biochem. Mol. Biol., 36, 299 - 304 (2003).
    A 056 0.6 ml, 2.5 ml, 6.0 ml  

    8- (6- Aminohexylamino)- 2'- O- methyladenosine- 3', 5'- cylic monophosphate, immobilized on agarose
       ( 8-AHA-2'-O-Me-cAMP-Agarose )
       Specific Epac agonist immobilized to agarose, Considered to be suitable for affinity chromatography of various cAMP-responsive
       proteins, especially those which tolerate modification of the ribose 2'-hydroxy group, such as the exchange protein activated by
       cyclic AMP (Epac) and certain phosphodiesterases
       Borland et al., Mol. Pharmacol., 69, 374 - 384 (2006).
    A 057 0.6 ml, 2.5 ml, 6.0 ml  

    2- (6- Aminohexylamino)adenosine- 3', 5'- cyclic monophosphate, immobilized on agarose
       ( 2-AHA-cAMP-Agarose )
       Cyclic AMP immobilized on agarose, used for affinity chromatography of various cyclic nucleotide-responsive proteins such as protein
       kinases, phosphodiesterases and others.
       Iwitzki, F., PhD Thesis, University of Bremen, Germany, 1993; Scholten et al., J. Proteome Res., 5, 1435 - 1447 (2006).
    A 054 0.6 ml, 2.5 ml, 6.0 ml  

    8- (6- Aminohexylamino)adenosine- 3', 5'- cyclic monophosphate, immobilized on agarose gel
       ( 8-AHA-cAMP Agarose )
       cyclic AMP immobilized on agarose, used for affinity chromatography of various cyclic nucleotide-responsive proteins such as protein
       kinases, phosphodiesterases and others.
       Dills et al., Biochemistry, 15, 3724 - 3730 (1976).
    A 028 0.6 ml, 2.5 ml, 6.0 ml  

    8- (6- Aminohexylamino)adenosine- 3', 5'- cyclic monophosphorothioate, Sp- isomer, immobilized on agarose gel
       ( Sp-8-AHA-cAMPS-Agarose )
       Immobilized PDE-resistant PKA agonist, used for affinity chromatography of various cyclic nucleotide-responsive proteins
       such as protein kinase, phosphodiesterases and others.
    A 013 0.6 ml, 2.5 ml, 6.0 ml  

    2- (6- Aminohexylamino)adenosine- 3', 5'- cyclic monophosphorothioate, Sp- isomer, immobilized to agarose gel
       ( Sp-2-AHA-cAMPS-Agarose )
       PDE-resistant Sp-cAMPS immobilized to agarose, used for affinity chromatography of various cyclic nucleotide-responsive proteins
       such as protein kinase, phosphodiesterases and others.
    A 013 0.6 ml, 2.5 ml, 6.0 ml  

    2- (6- Aminohexylamino)adenosine- 3', 5'- cyclic monophosphorothioate, Sp- isomer, immobilized to agarose gel
       ( Sp-2-AHA-cAMPS-Agarose )
       PDE-resistant Sp-cAMPS immobilized to agarose, used for affinity chromatography of various cyclic nucleotide-responsive proteins
       such as protein kinase, phosphodiesterases and others.
    A 069 0.6 ml, 2.5 ml, 6.0 ml  

    2'- (6- Aminohexylcarbamoyl)adenosine- 3', 5'- cyclic monophosphate, immobilized on agarose
       ( 2'-AHC-cAMP-Agarose )
       Cyclic AMP immobilized on agarose, used for affinity chromatography of various cyclic nucleotide-responsive proteins, especially those
       which tolerate modification of the 2'- ribose hydroxyl group such as some phosphodiesterases.
    A 058 0.6 ml, 2.5 ml, 6.0 ml  

    2'- (6- Aminohexylcarbamoyl)adenosine- 3', 5'- cyclic monophosphorothioate, Sp- isomer, immobilized on
       agarose gel ( Sp-2'-AHC-cAMPS-Agarose )
       PDE-resistant Sp-cAMPS immobilized on agarose , used for affinity chromatography of various cyclic nucleotide-responsive proteins,
       especially those which tolerate modification of the 2'- ribose hydroxyl group such as some phosphodiesterases.
    A 050 0.6 ml, 2.5 ml, 6.0 ml  

    2'- (6- Aminohexylcarbamoyl)guanosine- 3', 5'- cyclic monophosphate, immobilized on agarose
       ( 2'-AHC-cGMP-Agarose )
       Cyclic GMP immobilized on agarose, used for affinity chromatography of various cyclic nucleotide-responsive proteins, especially those
       which tolerate modification of the 2'- ribose hydroxyl group, such as some phosphodiesterases.
       Kim & Park, J. Biochem. Mol. Biol., 36, 299 - 304 (2003); Scholten et al., J. Proteome Res., 5, 1435 - 1447 (2006).
    A 059 0.6 ml, 2.5 ml, 6.0 ml  

    2'- (6- Aminohexylcarbamoyl)guanosine- 3', 5'- cyclic monophosphorothioate, Rp- isomer, immobilized on
       agarose gel ( Rp-2'-AHC-cGMPS-Agarose )
       PDE-resistant Rp-cGMPS immobilized to agarose, used for affinity chromatography of various cyclic nucleotide-responsive proteins,
       especially those which tolerate modification of the 2'- ribose hydroxyl group such as some phosphodiesterases.
       Corrie et al., Prot. Expr. Purif., 3, 417 - 420 (1992).
    A 051 0.6 ml, 2.5 ml, 6.0 ml  

    2'- (6- Aminohexylcarbamoyl)guanosine- 3', 5'- cyclic monophosphorothioate, Sp- isomer, immobilized on
       agarose gel ( Sp-2'-AHC-cGMPS-Agarose )
       PDE-resistant Sp-cGMPS immobilized to agarose , used for affinity chromatography of various cyclic nucleotide-responsive proteins,
       especially those which tolerate modification of the 2'- ribose hydroxyl group such as some phosphodiesterases.
       Corrie et al., Prot. Expr. Purif., 3, 417 - 420 (1992).
    A 052 0.6 ml, 2.5 ml, 6.0 ml  

    Ethanolamine immobilized on agarose ( EtOH-NH-Agarose )
       Control gel, Control gel without nucleotide ligand, deactivated with ethanolamine.
       Antl et al., Blood, 109, 552 - 559 (2007).
    E 010 0.6 ml, 2.5 ml, 6.0 ml