Modulators of CNG Ion Channels

  • Adenosine- 3' ,5'- cyclic monophosphate ( cAMP ), free acid formCat. No.: A 001 H
  • Adenosine- 3', 5'- cyclic monophosphate ( cAMP ), sodium saltCat. No.: A 001
  • 8- Bromo- ß- phenyl- 1, N²- ethenoguanosine- 3', 5'- cyclic monophosphorothioate, Rp- isomer ( Rp-8-Br-PET-cGMPS )Cat. No.: P 007
  • 8- Bromo- ß- phenyl- 1, N²- ethenoguanosine- 3', 5'-cyclic monophosphate ( 8-Br-PET-cGMP )Cat. No.: P 003
  • 8- Bromoadenosine- 3', 5'- cyclic monophosphate ( 8-Br-cAMP ), fluorescence gradeCat. No.: B 007
  • 8- Bromoguanosine- 3', 5'- cyclic monophosphate ( 8-Br-cGMP ), economy gradeCat. No.: B 004 E
  • 8- Bromoguanosine- 3', 5'- cyclic monophosphate ( 8-Br-cGMP ), fluorescence gradeCat. No.: B 004
  • 8- (4- Chlorophenylthio)- ß- phenyl- 1, N²- ethenoguanosine- 3', 5'- cyclic monophosphate ( 8-pCPT-PET-cGMP )Cat. No.: C 045
  • 8- (4- Chlorophenylthio)- ß- phenyl- 1, N²- ethenoguanosine- 3', 5'- cyclic monophosphorothioate, Sp- isomer ( Sp-8-pCPT-PET-cGMPS )Cat. No.: C 047
  • 8- (4- Chlorophenylthio)guanosine- 3', 5'- cyclic monophosphate ( 8-pCPT-cGMP ), economy gradeCat. No.: C 009 E
  • 8- (4- Chlorophenylthio)guanosine- 3', 5'- cyclic monophosphate ( 8-pCPT-cGMP ), fluorescence gradeCat. No.: C 009
  • 8- (4- Chlorophenylthio)guanosine- 3', 5'- cyclic monophosphorothioate, Rp- isomer ( Rp-8-pCPT-cGMPS )Cat. No.: C 013
  • 8- (4- Chlorophenylthio)guanosine- 3', 5'- cyclic monophosphorothioate, Sp- isomer ( Sp-8-pCPT-cGMPS )Cat. No.: C 014
  • 8- (2- [ DY-547]- aminoethylthio)guanosine- 3', 5'- cyclic monophosphate ( 8-[DY-547]-AET-cGMP )Cat. No.: D 087
  • 8- (2- [ DY-647]- aminoethylthio)guanosine- 3', 5'- cyclic monophosphate ( 8-[DY-647]-AET-cGMP )Cat. No.: D 090
  • 8- (2- [ Fluoresceinyl]aminoethylthio)adenosine- 3', 5'- cyclic monophosphate ( 8-Fluo-cAMP )Cat. No.: F 002
  • 8- (2- [ Fluoresceinyl]aminoethylthio)guanosine- 3', 5'- cyclic monophosphate ( 8-Fluo-cGMP )Cat. No.: F 001
  • Guanosine- 3', 5'- cyclic monophosphate ( cGMP )Cat. No.: G 001
  • β- Phenyl- 1, N²- ethenoguanosine- 3', 5'- cyclic monophosphate ( PET-cGMP )Cat. No.: P 001

     

  • Adenosine- 3' ,5'- cyclic monophosphate ( cAMP ), free acid form
       The second messenger cyclic AMP activates protein kinase A, cAMP-gated ion channels, HCN channels, the Epac receptor (cAMP-GEF), the CAP protein and
       some other target receptors. It is metabolized rapidly by cyclic nucleotide-dependent phosphodiesterases.
    A 001 H 500 mg/ ~1.5mM, 5 x 500 mg CAS No.: [60-92-4]
    Adenosine- 3' ,5'- cyclic monophosphate ( cAMP ), free acid form
       The second messenger cyclic AMP activates protein kinase A, cAMP-gated ion channels, HCN channels, the Epac receptor (cAMP-GEF), the CAP protein and
       some other target receptors. It is metabolized rapidly by cyclic nucleotide-dependent phosphodiesterases.
    A 001 500 uM/ ~176 mg, 5 x 500 uM CAS No.: [37839-81-9]

    8- Bromo- ß- phenyl- 1, N²- ethenoguanosine- 3', 5'- cyclic monophosphorothioate, Rp- isomer ( Rp-8-Br-PET-cGMPS )
       Selective and membrane-permeant inhibitor of both cyclic GMP dependent protein kinase I a and I ß and of retinal cGMP-gated ion channels. Resistant
       against mammalian cyclic nucleotide-dependent phosphodiesterases, no metabolic side effects.
       Butt et. al., Br. J. Pharmacol., 116, 3110 - 3116 (1995).
    P 007 1 uM/ ~0.6 mg, 5 x 1 uM CAS No.: [172806-20-1]

    8- Bromo- ß- phenyl- 1, N²- ethenoguanosine- 3', 5'-cyclic monophosphate ( 8-Br-PET-cGMP )
       Potent membrane-permeant activator of both isozymes I α and I ß of cGMP-dependent protein kinase while only a poor stimulator of type II. Common
       agonists such as 8-Br-cGMP or 8-pCPT-cGMP mainly activate kinase subtypes I α and II. 8-Br-PET-cGMP is one of the best activators of isozyme I ß and one
       of the best cyclic nucleotide muscle relaxants.
       Sekhar et al., Mol. Pharmacol., 42, 103 - 108 (1992).
    P 003 10 uM/ ~5.5 mg, 5 x 10 uM CAS No.: [144510-04-3]

    8- Bromoadenosine- 3', 5'- cyclic monophosphate ( 8-Br-cAMP ), fluorescence grade
       Activator of cAMP-dependent protein kinase and of Epac. Increased hydrolytical stability and membrane permeability compared to cAMP.
       Kroeber et al., J. Neurochem., 74, 2478 - 2489 (2000).
    B 007 100 uM/ ~43 mg, 5 x 100 uM CAS No.: [76939-46-3]

    8- Bromoguanosine- 3', 5'- cyclic monophosphate ( 8-Br-cGMP ), economy grade
       If fluorescent impurities disturb your assay, a specially purified quality is available ( B 004 Fluorescence Grade ) with a purity > 99% HPLC. Activator of
       cGMP-dependent protein kinases I α, I ß & type II and cGMP-gated ion channels (cyclic GMP agonist).
       Rapoport et al., Proc. Natl. Acad. Sci. USA, 79, 6470 - 6474 (1982).
    B 004 E 50 mg /~111 uM, 5 x 50 mg CAS No.: [51116-01-9]

    8- Bromoguanosine- 3', 5'- cyclic monophosphate ( 8-Br-cGMP ), fluorescence grade
       Activator of cGMP-dependent protein kinases I α, I ß & type II and cGMP-gated ion channels (cyclic GMP agonist). Increased metabolical stability compared to
       cGMP, but not completely stable as often stated.
       Rapoport et al., Proc. Natl. Acad. Sci. USA, 79, 6470 - 6474 (1982).
    B 004 50 uM /~22 mg , 5 x 50 uM CAS No.: [51116-01-9]

    8- (4- Chlorophenylthio)- ß- phenyl- 1, N²- ethenoguanosine- 3', 5'- cyclic monophosphate ( 8-pCPT-PET-cGMP )
       Highly membrane-permeant activator of cyclic GMP-dependent protein kinase, but most probably inhibitor of the retinal type cGMP-gated ion channel, thus
       suitable for distinction between kinase and ion channel mediated effects.
    C 045 5 uM /~3 mg , 5 x 5 uM CAS No.: [pending]

    8- (4- Chlorophenylthio)- ß- phenyl- 1, N²- ethenoguanosine- 3', 5'- cyclic monophosphorothioate, Sp- isomer
       ( Sp-8-pCPT-PET-cGMPS )
       Membrane-permeant activator of cyclic GMP-dependent protein kinase, but most probably inhibitor of the retinal type cGMP-gated ion channel, thus suitable
       for distinction between kinase and ion channel mediated effects. Resistant against mammalian cyclic nucleotide-dependent phosphodiesterases.
    C 047 1 uM /~0.6 mg , 5 x 1 uM CAS No.: [pending]

    8- (4- Chlorophenylthio)guanosine- 3', 5'- cyclic monophosphate ( 8-pCPT-cGMP ), economy grade
       If fluorescent impurities disturb your assay, a specially purified quality is available as well (C 009 Fluorescence Grade). Selective activator of both, cGMP-
      dependent protein kinase type I α and type II, as well as of cGMP-gated ion channels. Higher activation potential compared to cGMP; excellent cell membrane
       permeability and stability towards many phosphodiesterases.
       Geiger et al., Proc. Natl. Acad. Sci. USA, 89, 1031 - 1035 (1992).
    C 009 E 10 mg /~19 uM , 5 x 10 mg CAS No.: [51239-26-0]

    8- (4- Chlorophenylthio)guanosine- 3', 5'- cyclic monophosphate ( 8-pCPT-cGMP ), fluorescence grade
       Selective activator of both, cGMP-dependent protein kinase type I α and type II, as well as of cGMP-gated ion channels. Higher activation potential compared
       to cGMP; excellent cell membrane permeability and stability towards many phosphodiesterases. Recommendable alternative to unsuitable analogues such
       as dibutyryl cGMP.
       Geiger et al., Proc. Natl. Acad. Sci. USA, 89, 1031 - 1035 (1992).
    C 009 10 uM /~5 mg , 5 x 10 uM CAS No.: [51239-26-0]

    8- (4- Chlorophenylthio)guanosine- 3', 5'- cyclic monophosphorothioate, Rp- isomer ( Rp-8-pCPT-cGMPS )
       Potent selective inhibitor of protein kinase G I a, I ß and especially of type II. Activates retinal cGMP channels and thus discriminates between kinase and
       channel effects. Absolutely resistant against mammalian cyclic nucleotide-dependent phosphodiesterases, no metabolic side effects.
       Butt et al., Eur. J. Pharmacol., 269, 265 - 268 (1994); Gamm et al., J. Biol. Chem., 270, 27380 - 27388 (1996); Kramer et al., J. Neurosci., 16, 1285 - 1293 (1996).
    C 013 1 uM /~0.5 mg , 5 x 1 uM CAS No.: [153660-04-9]

    8- (4- Chlorophenylthio)guanosine- 3', 5'- cyclic monophosphorothioate, Sp- isomer ( Sp-8-pCPT-cGMPS )
       Activator of protein kinase G type I α and type II. Caution: Protein kinase A type II is activated as well. Excellent cell membrane permeability and phosphodi-
       esterase stability.
       Butt et al., Eur. J. Pharmacol., 269, 265 - 268 (1994), Gamm et al., J. Biol. Chem., 270, 27380 - 27388 (1996).
    C 014 5 uM /~2.6 mg , 5 x 5 uM CAS No.: [160385-87-5]

    8- (2- [ DY-547]- aminoethylthio)guanosine- 3', 5'- cyclic monophosphate ( 8-[DY-547]-AET-cGMP )
       Fluorescent cGMP analogue e.g. for research focussed on kinetic studies of CNG ion channels and other cGMP-reponsive binding proteins; λexc 557 nm,
       λem 574 nm.
       Biskup et al., Nature, 446, 440 - 443 (2007).
    D 087 0.1 uM /~0.1 mg , 5 x 0.1 uM CAS No.: [pending]

    8- (2- [ DY-647]- aminoethylthio)guanosine- 3', 5'- cyclic monophosphate ( 8-[DY-647]-AET-cGMP )
       Fluorescent cGMP analogue e.g. for research focussed on kinetic studies of CNG ion channels and other cGMP-reponsive binding proteins; λexc 649 nm,
       λem 666 nm. The fluorescent dye DY-647 conjugated with cyclic GMP
    D 090 0.1 uM /~0.1 mg , 5 x 0.1 uM CAS No.: [pending]

    8- (2- [ Fluoresceinyl]aminoethylthio)adenosine- 3', 5'- cyclic monophosphate ( 8-Fluo-cAMP )
       Fluorescent cAMP analogue; λexc 494 nm, λem 517 nm. cyclic AMP conjugated with fluorescein
       Schwede et al., Biochemistry, 39, 8803 - 8812 (2000).
    F 002 1 uM /~0.8 mg , 5 x 1 uM CAS No.: [293296-57-8]

    8- (2- [ Fluoresceinyl]aminoethylthio)guanosine- 3', 5'- cyclic monophosphate ( 8-Fluo-cGMP )
       Fluorescent cGMP analogue; λexc 494 nm, λem 517 nm.
       Tanaka et al, Biochemistry 28, 2776 - 2784 (1989); Koutalos et al., Biophys. J., 69, 2163 - 2167 (1995).
    F 001 1 uM /~0.8 mg , 5 x 1 uM CAS No.: [pending]

    Guanosine- 3', 5'- cyclic monophosphate ( cGMP )
       Target receptors for the second messenger cyclic GMP include protein kinase G isozymes, cGMP-gated ion channels as well as cGMP-responsive phosphodiesterases.
    G 001 100 uM /~37 mg , 5 x 100 uM CAS No.: [40732-48-7]

    β- Phenyl- 1, N²- ethenoguanosine- 3', 5'- cyclic monophosphate ( PET-cGMP )
       Potent membrane-permeant activator of both isozymes I α and I ß of cGMP-dependent protein kinase while only a poor stimulator of type II. Common
       agonists such as 8-Br-cGMP or 8-pCPT-cGMP mainly activate kinase subtypes I α and II.
       Gamm et al., J. Biol. Chem., 270, 27380 - 27388 (1996), Vaandrager et al., J. Biol. Chem., 272, 11816 - 11823 (1997).
    P 001 10 uM /~4.7 mg , 5 x 10 uM CAS No.: [78080-27-0]