Fluorescent Cyclic Nucleotides

  • 2- Aminopurine riboside- 3', 5'- monophosphate ( 2-NH2-cPuMP )Cat. No.: A 027
  • 2- Aza- 1, N6- ethenoadenosine- 3', 5'- cyclic monophosphate ( 2-Aza-ε-cAMP )Cat. No.: A 015
  • 8- Azido- 1, N6- ethenoadenosine- 3', 5'- cyclic monophosphate ( 8-N3-ε-cAMP )Cat. No.: A 077
  • 8- Azido- N6- (2- [DY-547]- aminoethyl)adenosine- 3', 5'- cyclic monophosphate ( 8-N3-6-[DY-547]-AE-cAMP )Cat. No.: A 100
  • 6, 7- Bis(carboxymethoxy)coumarin- 4- yl)methyl- adenosine- 3', 5'- cyclic monophosphate ( BCMCM-caged cAMP )Cat. No.: B 016
  • [6, 7- Bis(carboxymethoxy)coumarin- 4- yl]methyl- 8- bromoadenosine- 3', 5'- cyclic monophosphate ( BCMCM-caged 8-Br-cAMP )Cat. No.: B 018
  • [6, 7- Bis(carboxymethoxy)coumarin- 4- yl]methyl- 8- bromoguanosine- 3', 5'- cyclic monophosphate ( BCMCM-caged 8-Br-cGMP )Cat. No.: B 019
  • [6, 7- Bis(carboxymethoxy)coumarin- 4- yl]methyl- guanosine- 3', 5'- cyclic monophosphate ( BCMCM-caged cGMP )Cat. No.: B 017
  • 8- (2- [ DY-547]- aminoethylthio)- 2'- O- methyladenosine- 3', 5'- cyclic monophosphate ( 8-[DY-547]-AET-2'-O-Me-cAMP )Cat. No.: D 089
  • 8- (2- [ DY-547]- aminoethylthio)guanosine- 3', 5'- cyclic monophosphate ( 8-[DY-547]-AET-cGMP )Cat. No.: D 087
  • 8- (2- [ DY-647]- aminoethylthio)guanosine- 3', 5'- cyclic monophosphate ( 8-[DY-647]-AET-cGMP )Cat. No.: D 090
  • 1, N6- Ethenoadenosine- 3', 5'- cyclic monophosphate ( ε-cAMP )Cat. No.: E 001
  • 8- (2- [ Fluoresceinyl]aminoethylthio)adenosine- 3', 5'- cyclic monophosphate ( 8-Fluo-cAMP )Cat. No.: F 002
  • 8- (2- [ Fluoresceinyl]aminoethylthio)guanosine- 3', 5'- cyclic monophosphate ( 8-Fluo-cGMP )Cat. No.: F 001
  • 2'- (6- [ Fluoresceinyl]aminohexylcarbamoyl)adenosine- 3', 5'- cyclic monophosphate ( 2'-Fluo-AHC-cAMP )Cat. No.: F 003
  • 2'- (6- [ Fluoresceinyl]aminohexylcarbamoyl)guanosine- 3', 5'- cyclic monophosphate ( 2'-Fluo-AHC-cGMP )Cat. No.: F 004
  • 2'- O- (N- Methylanthraniloyl)adenosine- 3', 5'- cyclic monophosphate ( MANT-cAMP )Cat. No.: M 008
  • 2'- O- (N'- Methylanthraniloyl)cytidine- 3', 5'- cyclic monophosphate ( MANT-cCMP )Cat. No.: M 053
  • 2'- O- (N- Methylanthraniloyl)guanosine- 3', 5'- cyclic monophosphate ( MANT-cGMP )Cat. No.: M 009
  • 8- (4- [N- Methylanthraniloyl]aminobutylamino)adenosine- 3', 5'- cyclic monophosphate ( 8-MABA-cAMP )Cat. No.: M 016
  • N6- (6- [N- Methylanthraniloyl]hexylamino)adenosine-3', 5'- cyclic monophosphate ( 6-MAH-cAMP )Cat. No.: M 017
  • 8- (2- [7- Nitro- 4- benzofurazanyl]aminoethylthio)adenosine- 3', 5'- cyclic monophosphate ( 8-NBD-cAMP )Cat. No.: N 002
  • 8- (2- [7- Nitro- 4- benzofurazanyl]aminoethylthio)guanosine- 3', 5'- cyclic monophosphate ( 8-NBD-cGMP )Cat. No.: N 001
  • 8- [ Pharos-450]- adenosine- 3', 5'- cyclic monophosphate ( 8-[φ-450]-cAMP )Cat. No.: P 024
  • 8- [ Pharos-450]- guanosine- 3', 5'- cyclic monophosphate ( 8-[φ-450]-cGMP )Cat. No.: P 025
  • 8- [ Pharos-575]- 2'- O- methyladenosine- 3', 5'- cyclic monophosphate ( 8-[φ-575]-2'-O-Me-cAMP )Cat. No.: P 021
  • 8- [ Pharos-575]- adenosine- 3', 5'- cyclic monophosphate ( 8-[φ-575]-cAMP )Cat. No.: P 019
  • 8- [ Pharos-575]- guanosine- 3', 5'- cyclic monophosphate ( 8-[φ-575]-cGMP )Cat. No.: P 020

     

  • 2- Aminopurine riboside- 3', 5'- monophosphate ( 2-NH2-cPuMP )
       Fluorescent cyclic nucleotide.
       Scheit, J. Carbohydr. Nucleosides, Nucleotides, 1, 385 - 399 (1974).
    A 027 5 uM/ ~1.8 mg, 5 x 5 uM λexc 305 nm ;   λem 370 nm. CAS No.: [42467-66-3]

    2- Aza- 1, N6- ethenoadenosine- 3', 5'- cyclic monophosphate ( 2-Aza-ε-cAMP )
       Fluorescent cAMP analogue.
       Tsou et al., Anal. Biochem., 60, 163 - 169 (1974); Kraemer et al., J. Mol. Biol., 306, 1167 - 1177 (2001).
    A 015 5 uM/ ~1.9 mg, 5 x 5 uM λexc 358 nm ;   λem 494 nm. CAS No.: [50663-90-6]

    8- Azido- 1, N6- ethenoadenosine- 3', 5'- cyclic monophosphate ( 8-N3-ε-cAMP )
       Nonfluorescent cAMP analogue, that can be used for labelling of cAMP receptors. Upon UV-light-induced immobilization the ethenoadenine moiety starts to
       fluoresce, providing a label for tracing the receptor protein.
       Keeler & Campbell, Biochem. Biophys. Res. Commun., 72, 575 - 80 (1976); Dreyfuss et al., Proc. Nat. Acad. Sci. USA, 75, 1199 - 1203 (1978).
    A 077 1 uM/ ~0.4 mg, 5 x 1 uM   CAS No.: [60902-12-7]

    8- Azido- N6- (2- [DY-547]- aminoethyl)adenosine- 3', 5'- cyclic monophosphate ( 8-N3-6-[DY-547]-AE-cAMP )
       8-N3-6-[DY-547]-AE-cAMP is a light sensitive analogue for photoaffinity labelling. It can be immobilized within a cyclic AMP binding site by illumination with UV
       light. The fluorescent reporter group connected to position 6 of the adenine nucleobase can be excitated at 557 nm and emits with 574 nm.
       Keeler & Campbell, Biochem. Biophys. Res. Commun., 72, 575 - 80 (1976); Dreyfuss et al., Proc. Nat. Acad. Sci. USA, 75, 1199 - 1203 (1978).
    A 100 1 uM/ ~0.4 mg, 5 x 1 uM λexc 557 nm ;   λem 574 nm. CAS No.: [pending]

    6, 7- Bis(carboxymethoxy)coumarin- 4- yl)methyl- adenosine- 3', 5'- cyclic monophosphate ( BCMCM-caged cAMP )
       BCMCM-caged cAMP is an only weakly fluorescent, caged precursor of the second messenger cyclic AMP, which upon irradiation, releases both cAMP and a
       fluorescent coumarin analogue. Excellent solubility in water or buffers.
       Hagen et al., Angew. Chem. Int. Ed., 40, 1046 - 1048 (2001).
    B 016 0.1 uM/ ~68 ug, 5 x 0.1 uM   CAS No.: [339291-51-9]

    [6, 7- Bis(carboxymethoxy)coumarin- 4- yl]methyl- 8- bromoadenosine- 3', 5'- cyclic monophosphate ( BCMCM-caged 8-Br-cAMP )
       BCMCM-caged 8-Br-cAMP is an only weakly fluorescent, caged precursor of 8-bromo cyclic cAMP, which upon irradiation, releases both 8-Br-cAMP and a
       fluorescent coumarin analogue. Excellent solubility in water or buffers.
       Hagen et al., Angew. Chem. Int. Ed., 40, 1046 - 1048 (2001).
    B 018 0.1 uM/ ~76 ug, 5 x 0.1 uM   CAS No.: [370091-64-8]

    [6, 7- Bis(carboxymethoxy)coumarin- 4- yl]methyl- 8- bromoguanosine- 3', 5'- cyclic monophosphate ( BCMCM-caged 8-Br-cGMP )
       BCMCM-caged 8-Br-cGMP is an only weakly fluorescent, caged precursor of 8-bromo cyclic cGMP, which upon irradiation, releases both 8-Br-cGMP and a fluorescent coumarin analogue. Excellent solubility in water or buffers.
       Hagen et al., Angew. Chem. Int. Ed., 40, 1046 - 1048 (2001).
    B 019 0.1 uM/ ~78 ug, 5 x 0.1 uM   CAS No.: [370091-68-2]

    [6, 7- Bis(carboxymethoxy)coumarin- 4- yl]methyl- guanosine- 3', 5'- cyclic monophosphate ( BCMCM-caged cGMP )
       BCMCM-caged cGMP is an only weakly fluorescent, caged precursor of the second messenger cyclic cGMP, which upon irradiation, releases both cGMP
       and a fluorescent coumarin analogue. Excellent solubility in water or buffers.
       Hagen et al., Angew. Chem. Int. Ed., 40, 1046 - 1048 (2001).
    B 017 0.1 uM/ ~69 ug, 5 x 0.1 uM   CAS No.: [339291-52-0]

    8- (2- [ DY-547]- aminoethylthio)- 2'- O- methyladenosine- 3', 5'- cyclic monophosphate ( 8-[DY-547]-AET-2'-O-Me-cAMP )
       Fluorescent activator of the exchange protein directly activated by cyclic AMP (Epac) or cAMP-GEF, respectively, does not activate PKA.
    D 089 1 uM/ ~0.1 mg, 5 x 1 uM λexc 557 nm ;   λem 574 nm. CAS No.: [pending]

    8- (2- [ DY-547]- aminoethylthio)guanosine- 3', 5'- cyclic monophosphate ( 8-[DY-547]-AET-cGMP )
       Fluorescent cGMP analogue e.g. for research focussed on kinetic studies of CNG ion channels and other cGMP-reponsive binding proteins.
       Biskup et al., Nature, 446, 440 - 443 (2007).
    D 087 1 uM/ ~0.1 mg, 5 x 1 uM λexc 557 nm ;   λem 574 nm. CAS No.: [pending]

    8- (2- [ DY-647]- aminoethylthio)guanosine- 3', 5'- cyclic monophosphate ( 8-[DY-647]-AET-cGMP )
       Fluorescent cGMP analogue e.g. for research focussed on kinetic studies of CNG ion channels and other cGMP-reponsive binding proteins. The fluorescent
       dye DY-647 conjugated with cyclic GMP.
    D 090 1 uM/ ~0.1 mg, 5 x 1 uM λexc 649 nm ;   λem 666 nm. CAS No.: [pending]

    1, N6- Ethenoadenosine- 3', 5'- cyclic monophosphate ( ε-cAMP )
       Fluorescent cAMP analogue.
       Secrist et al., Biochemistry, 11, 3499 - 3506 (1972).
    E 001 10 uM/ ~3.8 mg, 5 x 10 uM λexc 300 nm ;   λem 415 nm. CAS No.: [103213-51-0]

    8- (2- [ Fluoresceinyl]aminoethylthio)adenosine- 3', 5'- cyclic monophosphate ( 8-Fluo-cAMP )
       cyclic AMP conjugated with fluorescein.
       Schwede et al., Biochemistry, 39, 8803 - 8812 (2000).
    F 002 1 uM/ ~0.8 mg, 5 x 1 uM λexc 494 nm ;   λem 517 nm. CAS No.: [293296-57-8]

    8- (2- [ Fluoresceinyl]aminoethylthio)guanosine- 3', 5'- cyclic monophosphate ( 8-Fluo-cGMP )
       Fluorescent cyclic GMP analogue.
       Tanaka et al, Biochemistry 28, 2776 - 2784 (1989); Koutalos et al., Biophys. J., 69, 2163 - 2167 (1995).
    F 001 1 uM/ ~0.8 mg, 5 x 1 uM λexc 494 nm ;   λem 517 nm. CAS No.: [pending]

    2'- (6- [ Fluoresceinyl]aminohexylcarbamoyl)adenosine- 3', 5'- cyclic monophosphate ( 2'-Fluo-AHC-cAMP )
       Fluorescent, PKA-inactive cyclic AMP analogue. Suitable for phosphodiesterase studies.
       Huang et al., J. Biomol. Screen., 7, 215 - 222 (2002).
    F 003 1 uM/ ~0.9 mg, 5 x 1 uM λexc 494 nm ;   λem 517 nm. CAS No.: [pending]

    2'- (6- [ Fluoresceinyl]aminohexylcarbamoyl)guanosine- 3', 5'- cyclic monophosphate ( 2'-Fluo-AHC-cGMP )
       Fluorescent, PKG-inactive cyclic GMP analogue. Suitable for phosphodiesterase studies.
       Huang et al., J. Biomol. Screen., 7, 215 - 222 (2002).
    F 004 1 uM/ ~0.9 mg, 5 x 1 uM λexc 494 nm ;   λem 517 nm. CAS No.: [pending]

    2'- O- (N- Methylanthraniloyl)adenosine- 3', 5'- cyclic monophosphate ( MANT-cAMP )
       Fluorescent cyclic AMP analogue, suitable for phosphodiesterase studies.
       Hiratsuka, J. Biol. Chem., 257, 13354 - 13358 (1982); Kraemer et al., J. Mol. Biol., 306, 1167 - 1177 (2001).
    M 008 10 uM/ ~4.8 mg, 5 x 10 uM λexc 350 nm ;   λem 445 nm. CAS No.: [83707-14-6]

    2'- O- (N'- Methylanthraniloyl)cytidine- 3', 5'- cyclic monophosphate ( MANT-cCMP )
       Fluorescent, membrane-permeant cyclic CMP analogue, potentially suitable for phosphodiesterase studies. The compound is expected to show a decrease
       in fluorescence when cleaved by PDE.
       Hiratsuka, J. Biol. Chem., 257, 13354 - 13358 (1982).
    M 053 5 uM/ ~2.3 mg, 5 x 5 uM λexc 350 nm ;   λem 441 nm. CAS No.: [pending]

    2'- O- (N- Methylanthraniloyl)guanosine- 3', 5'- cyclic monophosphate ( MANT-cGMP )
       Fluorescent cyclic GMP analogue, suitable for phosphodiesterase studies. The compound shows a decrease in fluorescence when cleaved by PDE.
       Hiratsuka, J. Biol. Chem., 257, 13354 - 13358 (1982); Johnson, Anal. Biochem., 162, 291 - 295 (1987); Brown et al., Biochem. Biophys. Res. Commun., 224, 684 - 689 (1996).
    M 009 10 uM/ ~5 mg, 5 x 10 uM λexc 350 nm ;   λem 441 nm. CAS No.: [83707-15-7]

    8- (4- [N- Methylanthraniloyl]aminobutylamino)adenosine- 3', 5'- cyclic monophosphate ( 8-MABA-cAMP )
       Fluorescent, membrane- permeant cyclic AMP analogue.
       Kraemer et al., J. Mol. Biol., 306, 1167 - 1177 (2001).
    M 016 5 uM/ ~2.9 mg, 5 x 5 uM λexc 352 nm ;   λem 436 nm. CAS No.: [342029-95-2]

    N6- (6- [N- Methylanthraniloyl]hexylamino)adenosine-3', 5'- cyclic monophosphate ( 6-MAH-cAMP )
       Fluorescent, membrane- permeant cyclic AMP analogue.
       Kraemer et al., J. Mol. Biol., 306, 1167 - 1177 (2001); Moll et al., J. Neural. Transm., 113, 1015 - 1032 (2006). Theyssen, J. Mol. Biol., 263, 657 - 670 (1996).
    M 017 5 uM/ ~2.9 mg, 5 x 5 uM λexc 333nm ;   λem 437 nm. CAS No.: [723313-27-7]

    8- (2- [7- Nitro- 4- benzofurazanyl]aminoethylthio)adenosine- 3', 5'- cyclic monophosphate ( 8-NBD-cAMP )
       Potent membrane-permeant fluorescent activator of cAMP-dependent protein kinase. While 8-NBD-cAMP is almost non-fluorescent in aqueous solutions, it
       fluoresces strongly in hydrophobic environments such as hydrophobic protein binding sites.
       Kraemer et al., J. Mol. Biol., 306, 1167 - 1177 (2001).
    N 002 1 uM/ ~0.6 mg, 5 x 1 uM λexc 471nm ;   λem 536 nm. CAS No.: [221905-51-7]

    8- (2- [7- Nitro- 4- benzofurazanyl]aminoethylthio)guanosine- 3', 5'- cyclic monophosphate ( 8-NBD-cGMP )
       Potent membrane-permeant fluorescent activator of both isozymes I a and I ß of cGMP-dependent protein kinase. While 8-NBD-cGMP is almost
       nonfluorescent in aqueous solutions, it fluoresces strongly in hydrophobic environments such as hydrophobic protein binding sites.
       Ruf et al. in Heilmeyer (ed.), Signal Transduction and Protein Phosphorylation, Ser. A, Life Sci., 135, 99 - 104 ( 1987), Plenum Press, NATO ASI.
    N 001 1 uM/ ~0.7 mg, 5 x 1 uM λexc 475nm ;   λem 525 nm. CAS No.: [115993-88-9]

    8- [ Pharos-450]- adenosine- 3', 5'- cyclic monophosphate ( 8-[φ-450]-cAMP )
       cyclic AMP connected to the relatively small Pharos 450 fluorophore (λexc 450 nm, λem 534 nm) by a spacer. The fluorophore can be excited e.g. by a He/Cd
       laser. Due to its high lipophilicity and bright green fluorescence 8-[φ-450]-cAMP is especially suitable for studies with intact cells.
    P 024 0.1 uM/ ~ 72 ug, 5 x 0.1 uM λexc 450nm ;   λem 534 nm. CAS No.: [pending]

    8- [ Pharos-450]- guanosine- 3', 5'- cyclic monophosphate ( 8-[φ-450]-cGMP )
       cyclic GMP connected to the relatively small Pharos 450 fluorophore. The fluorophore can be excited e.g. by a He/Cd laser. Due to its high lipophilicity and
       bright green fluorescence 8-[φ-450]-cGMP is especially suitable for studies with intact cells.
    P 025 0.1 uM/ ~ 74 ug, 5 x 0.1 uM λexc 450nm ;   λem 534 nm. CAS No.: [pending]

    8- [ Pharos-575]- 2'- O- methyladenosine- 3', 5'- cyclic monophosphate ( 8-[φ-575]-2'-O-Me-cAMP )
       2’-O-Me-cAMP connected to the relatively small Pharos 575 dye . The fluorophore can be excited e.g. by a Kr/Ar laser. Due to its high lipophilicity and bright
       red fluorescence 8-[φ-575]-cAMP is especially suitable for studies with intact cells.
    P 021 0.1 uM/ ~ 74 ug, 5 x 0.1 uM λexc 577nm ;   λem 605 nm. CAS No.: [pending]

    8- [ Pharos-575]- adenosine- 3', 5'- cyclic monophosphate ( 8-[φ-575]-cAMP )
       cyclic AMP connected to the relatively small Pharos 575 dye by a spacer. The fluorophore can be excited e.g. by a Kr/Ar laser. Due to its high lipophilicity and
       bright red fluorescence 8-[φ-575]-cAMP is especially suitable for studies with intact cells.
    P 019 0.1 uM/ ~ 72 ug, 5 x 0.1 uM λexc 577nm ;   λem 605 nm. CAS No.: [pending]

    8- [ Pharos-575]- guanosine- 3', 5'- cyclic monophosphate ( 8-[φ-575]-cGMP )/TD>
       cyclic GMP connected to the relatively small Pharos 575 dye . The fluorophore can be excited e.g. by a Kr/Ar laser. Due to its high lipophilicity and bright red
       fluorescence 8-[φ-575]-cGMP is especially suitable for studies with intact cells.
    P 020 0.1 uM/ ~ 74 ug, 5 x 0.1 uM λexc 577nm ;   λem 605 nm. CAS No.: [pending]