TAQ DNA Polymerase
Cat. No. Enzyme. no buffer included
  A05xxxx Taq DNA Polymerase 1 U/ul
  A06xxxx Taq DNA Polymerase 1 U/ul RED
  A10xxxx Taq DNA Polymerase 5 U/ul glycerol free
  A11xxxx Taq DNA Polymerase 5 U/ul
  A20xxxx Taq DNA Polymerase 5 U/ul RED
Cat. No. Units Volume of enzyme 1U/ul Volume of enzyme 5U/ul
  Axx0002 250 250ul 50ul
  Axx0003 500 500ul 100ul
  Axx0004 1000 2 x 500ul 2 x 100ul
  Axx0006 2500 5 x 500ul 5 x 100ul
  Axx0007 5000 8 x 1.25 ml 10 x 100ul
  Axx0008 10000 16 x 1.25 ml 3 x 667 ul
 

TAQ DNA Polymerase KITS (I)
Cat. No. Enzyme. one buffer and MgCl2
  A05xxxx Taq DNA Polymerase 1 U/ul
  A06xxxx Taq DNA Polymerase 1 U/ul RED
  A10xxxx Taq DNA Polymerase 5 U/ul glycerol free
  A11xxxx Taq DNA Polymerase 5 U/ul
  A20xxxx Taq DNA Polymerase 5 U/ul RED
Cat. No. Buffer (MgCl2 if not MG2+ free)
  Axx11xx 10 x Ammonium Buffer
  Axx12xx 10 x Ammonium Buffer, Mg2+ free
  Axx14xx 10 x Ammonium Buffer, Tween free
  Axx15xx 10 x Ammonium Buffer, Mg2+ free and Tween free
  Axx21xx 10 x Standard Buffer
  Axx22xx 10 x Standard Buffer, Mg2+ free
  Axx24xx 10 x Standard Buffer, Tween free
  Axx25xx 10 x Standard Buffer, Mg2+ free and Tween free
  Axx31xx 10 x Combination Buffer
  Axx32xx 10 x Combination Buffer, Mg2+ free
  Axx34xx 10 x Combination Buffer, Tween free
  Axx35xx 10 x Combination Buffer, Mg2+ free and Tween free
  Cat. No. Units Volume of enzyme 1U/ul Volume of enzyme 5U/ul Volume of buffer Volume of 25mM MgCl2
  Axxxx02 250 250ul 50ul 1.5ml 1.5ml
  Axxxx03 500 500ul 100ul 1.5ml 1.5ml
  Axxxx04 1000 2 x 500ul 2 x 100ul 2 x 1.5ml 2 x 1.5ml
  Axxxx06 2500 5 x 500ul 5 x 100ul 4 x 1.5ml 4 x 1.5ml
  Axxxx07 5000 8 x 1.25ml 10 x 100ul 3 x 5ml 3 x 5ml
  Axxxx08 10000 16 x 1.25ml 3 x 667ul 3 x 5ml 6 x 5ml
 

TAQ DNA Polymerase KITS (II)
Cat. No. Enzyme. two buffer and MgCl2
  A05xxxx Taq DNA Polymerase 1 U/ul
  A06xxxx Taq DNA Polymerase 1 U/ul RED
  A10xxxx Taq DNA Polymerase 5 U/ul glycerol free
  A11xxxx Taq DNA Polymerase 5 U/ul
  A20xxxx Taq DNA Polymerase 5 U/ul RED
Cat. No. Buffer (MgCl2 if not MG2+ free)
  Axx41xx 10 x Ammonium Buffer and 10 x Standard Buffer
  Axx42xx 10 x Ammonium Buffer and 10 x Standard Buffer both Mg2+ free
  Axx44xx 10 x Ammonium Buffer and 10 x Standard Buffer both detergent free
  Axx45xx 10 x Ammonium Buffer and 10 x Standard Buffer both Mg2+ free and detergent free
  Axx51xx 10 x Ammonium Buffer and 10 x Combination Buffer
  Axx52xx 10 x Ammonium Buffer and 10 x Combination Buffer both Mg2+ free
  Axx54xx 10 x Ammonium Buffer and 10 x Combination Buffer both detergent free
  Axx55xx 10 x Ammonium Buffer and 10 x Combination Buffer both Mg2+ free and detergent free
  Cat. No. Units Volume of enzyme 1U/ul Volume of enzyme 5U/ul Volume of buffers Volume of 25mM MgCl2
  Axxxx02 250 250ul 50ul (1+1) x 1.5ml 1.5ml
  Axxxx03 500 500ul 100ul (1+1) x 1.5ml 1.5ml
  Axxxx04 1000 2 x 500ul 2 x 100ul (2+2) x 1.5ml 2 x 1.5ml
  Axxxx06 2500 5 x 500ul 5 x 100ul (4+4) x 1.5ml 4 x 1.5ml
  Axxxx07 5000 8 x 1.25ml 10 x 100ul (3+3) x 5ml 3 x 5ml
  Axxxx08 10000 16 x 1.25ml 3 x 667ul (6+6) x 5ml 6 x 5ml
 

TAQ DNA Polymerase Master Mix and Master Mix RED
  One reaction in final volume with Ampliqon Master Mixes is defined as 50 μl containing 5 units of the respective polymerase.
Cat. No. 1.1 x Master Mix
  A1203xx Taq DNA Polymerase 1.1x Master Mix, 1.5 mM MgCl2 (final concentration)
  A1303xx Taq DNA Polymerase 1.1x Master Mix, 2.0 mM MgCl2 (final concentration)
  A1603xx Taq DNA Polymerase 1.1x Master Mix RED, 1.5 mM MgCl2 (final concentration)
  A1703xx Taq DNA Polymerase 1.1x Master Mix RED, 2.0 mM MgCl2 (final concentration)
  Cat. No. Units Volume of Master Mix
  Axxxx01 100 3 x 1.5ml
  Axxxx03 500 15 x 1.5ml
  Axxxx06 2500 75 x 1.5ml
  Axxxx07 5000 45 x 5ml
  Axxxx08 10000 45 x 10ml
  Axxxx09 20000 1 x 900ml
 

TAQ DNA POLYMERASE MASTER MIX AND MASTER MIX RED
Cat. No. 2 x Master Mix
  A1403xx Taq DNA Polymerase 2 x Master Mix, 1.5 mM MgCl2 (final concentration)
  A1503xx Taq DNA Polymerase 2 x Master Mix, 2.0 mM MgCl2 (final concentration)
  A1803xx Taq DNA Polymerase 2 x Master Mix RED, 1.5 mM MgCl2 (final concentration)
  A1903xx Taq DNA Polymerase 2 x Master Mix RED, 2.0 mM MgCl2 (final concentration)
  Cat. No. Units Volume of Master Mix
  Axxxx01 100 2 x 1.25ml
  Axxxx03 500 10 x 1.25ml
  Axxxx06 2500 50 x 1.25ml
  Axxxx07 5000 25 x 5ml
  Axxxx08 10000 25 x 10ml
  Axxxx09 20000 1 x 500ml
 

TEMPase HOT START POLYMERASE
Cat. No. Enzyme, no buffer
  A22xxxx TEMPase Hot Start DNA Polymerase 5U/ul
  Cat. No. Units Volume of Master Mix
  A220002 250 50 ul
  A220003 500 100ul
  A220004 1000 2 x 100ul
  A220006 2500 5 x 100ul
  A220007 5000 10 x 100ul
  A220008 10000 3 x 667ul
 

TEMPase HOT START POLYMERASE KITS
Cat. No. Enzyme, one buffer and MgCl2
  A22xxxx TEMPase Hot Start DNA Polymerase 5U/ul
Cat. No. Buffer ( 15mM MgCl2 if not Mg2+ free)
  A2211xx 10 x Ammonium Buffer
  A2212xx 10 x Ammonium Buffer, Mg2+ free
  A2214xx 10 x Ammonium Buffer, Tween free
  A2215xx 10 x Ammonium Buffer, Mg2+ free and Tween free
  A2231xx 10 x Combination Buffer
  A2232xx 10 x Combination Buffer Mg2+ free
  A2234xx 10 x Combination Buffer, Tween free
  A2235xx 10 x Combination Buffer, Mg2+ free and Tween free
  Cat. No. Units Volume of enzyme Volume of buffers Volume of 25mM MgCl2
  A22xx02 250 50ul 1.5ml 1.5ml
  A22xx03 500 100ul 1.5ml 1.5ml
  A22xx04 1000 2 x 100ul 2 x 1.5ml 2 x 1.5ml
  A22xx06 2500 5 x 100ul 4 x 1.5ml 4 x 1.5ml
  A22xx07 5000 10 x 100ul 3 x 5ml 3 x 5ml
  A22xx08 10000 3 x 667ul 6 x 5ml 6 x 5ml
 

TEMPase HOT START POLYMERASE KITS
Cat. No. Enzyme. two buffer and MgCl2
  A22xxxx TEMPase Hot Start DNA Polymerase 5U/ul
Cat. No. Buffer
  A2251xx 10 x Ammonium Buffer and 10 x Combination Buffer
  A2252xx 10 x Ammonium Buffer and 10 x Combination Buffer both Mg2+ free
  A2254xx 10 x Ammonium Buffer and 10 x Combination Buffer both detergent free
  A2255xx 10 x Ammonium Buffer and 10 x Combination Buffer both Mg2+ free and detergent free
  Cat. No. Units Volume of enzyme Volume of buffers Volume of 25mM MgCl2
  A22xx02 250 50ul (1+1) x 1.5ml 1.5ml
  A22xx03 500 100ul (1+1) x 1.5ml 1.5ml
  A22xx04 1000 2 x 100ul (2+2) x 1.5ml 2 x 1.5ml
  A22xx06 2500 5 x 100ul (4+4) x 1.5ml 4 x 1.5ml
  A22xx07 5000 10 x 100ul (3+3) x 5ml 3 x 5ml
  A22xx08 10000 3 x 667ul (6+6) x 5ml 6 x 5ml
 

TEMPase HOT START MASTER MIX AND MASTER MIX BLUE
  One reaction in final volume with Ampliqon Master Mixes is defined as 50 μl containing 5 units of the respective polymerase.
Cat. No. Hot Start 2 x Master Mix
  A2303xx TEMPase Hot Start 2x Master Mix I 1.5 mM MgCl2 (final concentration)
  A2307xx TEMPase Hot Start 2x Master Mix II 1.5 mM MgCl2 (final concentration)
  A2904xx TEMPase Hot Start 2x Master Mix BLUE I 1.5 mM MgCl2 (final concentration)
  A2908xx TEMPase Hot Start 2x Master Mix BLUE II 1.5 mM MgCl2 (final concentration)
  Cat. No. Reactions Volume of Master Mix
  Axxxx01 100 2 x 1.25ml
  Axxxx03 500 10 x 1.25ml
  Axxxx04 1000 20 x 1.25ml
  Axxxx06 2500 50 x 1.25ml
  Axxxx07 5000 25 x 5ml
  Axxxx08 10000 25 x 10ml
  Axxxx09 20000 1 x 500ml
 

REAL TIME MASTER MIX
Cat. No. RealQ PCR 2x Master Mix
  A3107xx RealQ PCR 2x Master Mix 7 mM MgCl2, for probe (no ROX)
  A3117xx RealQ PCR 2x Master Mix 7 mM MgCl2, for probe (low ROX)
  A3127xx RealQ PCR 2x Master Mix 7 mM MgCl2, for probe (high ROX)
  A3207xx RealQ PCR 2x Master Mix 7 mM MgCl2, with green dye (no ROX)
  A3217xx RealQ PCR 2x Master Mix 7 mM MgCl2, with green dye (low ROX)
  A3227xx RealQ PCR 2x Master Mix 7 mM MgCl2, with green dye (high ROX)
  Cat. No. Reactions Volume of Master Mix
  Axxxx01 200 4 x 1.25ml
  Axxxx05 500 10 x 5ml
 

HIGH FIDELITY - THERMOSTABLE PROOFREADER
Cat. No. High fidelity products, no buffer included
  A21xxxx AccuPOL DNA Polymerase
  Cat. No. Units Volume of enzyme
  A210002 250 100ul
  A210003 500 200ul
  A210004 1000 2 x 200ul
  A210006 2500 5 x 200ul
 

HIGH FIDELITY - THERMOSTABLE PROOFREADER - KITS
Cat. No. Enzyme, one buffer and MgCl2
  A21xxxx AccuPOL DNA Polymerase
Cat. No. Buffer (15 mM MgCl2 if not Mg2+ free)
  A2111xx 10x Ammonium Buffer
  A2112xx 10x Ammonium Buffer, Mg2+ free
  A2114xx 10x Ammonium Buffer, Tween free
  A2115xx 10x Ammonium Buffer, Mg2+ free and Tween free
  Cat. No. Units Volume of enzyme Volume of buffers Volume of 25mM MgCl2
  A21xx02 250 100ul 1.5ml 1.5ml
  A21xx03 500 200ul 1.5ml 1.5ml
  A21xx04 1000 2 x 200ul 2 x 1.5ml 2 x 1.5ml
  A21xx06 2500 5 x 200ul 4 x 1.5ml 4 x 1.5ml
 

WHOLE GENOME AMPLIFICATION KIT
  One reaction in final volume with Ampliqon Master Mixes is defined as 50 μl containing 5 units of the respective polymerase.
Cat. No. Enzyme, buffer, primers and control DNA
  A2808xx AmpliQ Genomic Amplifier Kit
  Cat. No. Reactions Volume of Amplifier Enzyme Volume of 5 x Amplifier Buffer(dNTP Included) Volume of Primer Mix Volume of Control Genomic DNA (10ng/ul)
  A280801 25 25ul 250ul 60ul 30ul
  A280803 100 100ul 1ml 200ul 100ul
  A280805 500 500ul 5 x 1ml 1ml 500ul
 

BUFFERS, MgCl2 AND ENHANCER
  Cat. No. Products Volume
  A301110   10x Ammonium Buffer 10 x 1.5 ml
  A301210   10x Ammonium Buffer, Mg2+ free 10 x 1.5 ml
  A301410   10x Ammonium Buffer, Tween free 10 x 1.5 ml
  A301510   10x Ammonium Buffer, Mg2+ free and Tween free 10 x 1.5 ml
  A302110   10x Standard Buffer 10 x 1.5 ml
  A302210   10x Standard Buffer, Mg2+ free 10 x 1.5 ml
  A302410   10x Standard Buffer, Triton free 10 x 1.5 ml
  A302510   10x Standard Buffer, Mg2+ free and Triton free 10 x 1.5 ml
  A303110   10x Combination Buffer 10 x 1.5 ml
  A303210   10x Combination Buffer, Mg2+ free 10 x 1.5 ml
  A303410   10x Combination Buffer, Tween free? 10 x 1.5 ml
  A303510   10x Combination Buffer, Mg2+ free and Tween free 10 x 1.5 ml
  A308103   25 mM MgCl2 3 x 1.5 ml
  A308110   25 mM MgCl2 10 x 1.5 ml
  A351104   Betaine Enhancer solution 5 M 5 x 1 ml
  A351413   50x Glass Blocking agent 3 x 0.2 ml
  A351423   50x Glass Blocking agent 3 x 1.25 ml
  A351513   ROX internal reference dye 3 x 0.2 ml
 

dNTP MIX
  Cat. No. Size dNTP Mix Concentration of each nucleotide
  A500004   100uM 2 x 500ul 25 mM
  A500007   400uM 8 x 500ul 25 mM
  A501004   50uM 2 x 500ul 12.5 mM
  A501007   200uM 8 x 500ul 12.5 mM
  A502004   40uM 2 x 500ul 10 mM
  A502007   160uM 8 x 500ul 10 mM
 

dNTP SET
  Cat. No. Size 100 mM dATP 100 mM dCTP 100 mM dGTP 100 mM dTTP
  A511104   25uM 1 x 250ul 1 x 250ul 1 x 250ul 1 x 250ul
  A511107   100uM 4 x 250ul 4 x 250ul 4 x 250ul 4 x 250ul
  A511109   500uM 20 x 250ul 20 x 250ul 20 x 250ul 20 x 250ul
  A511120   200uM 2 x 1ml 2 x 1ml 2 x 1ml 2 x 1ml
 

SINGLE dNTP
  Cat. No. dNTP Size Volume Concentration of nucleotide
  A521102   dATP 25 uM 1 x 250ul 100mM
  A521202   dCTP 25 uM 1 x 250ul 100mM
  A521302   dGTP 25 uM 1 x 250ul 100mM
  A521402   dTTP 25 uM 1 x 250ul 100mM